1H High Resolution Magic-Angle Coil Spinning (HR-MACS) µNMR Metabolic Profiling of whole Saccharomyces cervisiae cells: A Demonstrative Study

1H High Resolution Magic-Angle Coil Spinning (HR-MACS) µNMR Metabolic Profiling of whole Saccharomyces cervisiae cells: A Demonstrative Study

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The low sensitivity of Nuclear Magnetic Resonance (NMR) is its prime shortcoming compared to here other analytical methods for metabolomic studies.It relies on large sample volume (30–50 µl for HR-MAS) for rich metabolic profiling, hindering high-throughput screening especially when the sample requires a labor-intensive preparation or is a sacred specimen.This is indeed the case for some living organisms.

This study Jeans evaluates a 1H HR-MAS approach for metabolic profiling of small volume (250 nl) whole bacterial cells, Saccharomyces cervisiae, using an emerging micro-NMR technology: high-resolution magic-angle coil spinning (HR-MACS).As a demonstrative study for whole cells, we perform two independent metabolomics studies identifying the significant metabolites associated with osmotic stress and aging.